Fig 1: Normal expression and phosphorylation of the IL-2 receptor and its downstream signaling components. PBLs of healthy individuals or sorted ascites-derived CD4+CD25− T cells (5 × 105 cells/well) were stimulated with 20,000 αCD3/28 beads/well for three days. Subsequently, samples were stained for flow cytometry analysis. (A) Cells were stained for isotype control, CD25, CD122, and CD132. Plots are representative for eight controls and three patients. (B) Control (n = 3) and ascites-derived T cells (n = 3) were stained for JAK3, STAT5, phospho-STAT5, phosphor-AKT and phosphor-ERK. One representative example is shown.